Coding

Part:BBa_J37023:Design

Designed by: John Chattaway   Group: iGEM06_Imperial   (2006-07-27)


Immunologically Tagged aiiA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The FLAG had to be added at the 5' end of the sequence this was done using PCR


Source

[1]

References